von Willebrand Factor Antibody / vWF

• Catalog Number: orb1151862
• Category: Antibodies
• Description: Mouse monoclonal antibody to von Willebrand Factor
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 1800-2000 was used as the immunogen for the von Willebrand Factor antibody.
• UniProt ID: P04275
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the von Willebrand Factor antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: VWF/4106
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This von Willebrand Factor antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with von Willebrand Factor antibody (clone VWF/4106). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human pancreatic tissue with von Willebrand Factor antibody (clone VWF/4106). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free von Willebrand Factor antibody (VWF/4106) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using von Willebrand Factor antibody (clone VWF/4106). These results demonstrate the foremost specificity of the VWF/4106 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.