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Catalog Number | orb1259469 |
---|---|
Category | Antibodies |
Description | VMP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human VMP1 (NP_112200.2). |
Concentration | batch dependent |
Dilution range | WB: 1:200 - 1:2000IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 42kDa |
Target | VMP1 |
UniProt ID | Q96GC9 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Vacuole membrane protein 1, Transmembrane protein Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:2000IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of mouse kidney, using VMP1 antibody (orb1259469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunofluorescence analysis of NIH-3T3 cells using VMP1 Polyclonal Antibody (orb1259469) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using VMP1 Polyclonal Antibody (orb1259469) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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