VMAT2 Antibody

• Catalog Number: orb1247892
• Category: Antibodies
• Description: VMAT2 Antibody
• Target: SLC18A2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-SYPIGEDEESESD
• UniProt ID: Q05940
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Placenta and Adrenal Gland lysates (calculated MW of 55.7kDa according to NP_003045.2). Recommended concentration 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml. In paraffin embedded Human Adrenal Gland expression of SLC18A2 is detected in chromaffin cells. Recommended concentration: 2.5ug/ml
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SLC18A2, VMAT2, SVAT, SVMT, VAT2, solute carrier f
• Research Area: Neuroscience
• Note: For research use only
• NCBI: NP_003045.2
• Expiration Date: 12 months from date of receipt.

orb1247892 (1 ug/ml) staining of Human Placenta (A) and Human Adrenal Gland (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247892 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247892 (2.5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247892 Negative Control showing staining of paraffin embedded Human Adrenal Gland, with no primary antibody.