VISTA Antibody [4C4]

• Catalog Number: orb1240290
• Category: Antibodies
• Description: VISTA Antibody [4C4]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4C4
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: VISTA antibody was raised against the extracellular domain of human VISTA.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 31 kDa Observed: 47 kDa
• Target: VSIR
• UniProt ID: Q9H7M9
• NCBI: NP_071436
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5,
• Note: For research use only
• Application notes: VISTA antibody can be used for detection of VISTA by Western blot at 0.25 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of VISTA in overexpressing HEK293 cells with VISTA antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml

Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.

Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL. Green: VISTA Antibody [4C4] (orb1240290) Blue: DAPI staining

Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 5 μg/mL. Red: VISTA Antibody [4C4] (orb1240290) Blue: DAPI staining

Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL. Red: VISTA Antibody [4C4] (orb1240290) Blue: DAPI staining

Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.

Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.

Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.