NAMPT Antibody

SKU: orb1272636

Description

NAMPT Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human Visfatin. Human Visfatin specific antibody was purified by affinity chromatography employing an immobilized Human Visfatin matrix.
• Target: NAMPT
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VF, PBEF, PBEF1, VISFATIN, 1110035O14Rik, Nicotinamide phosphoribosyltransferase, Pre-B-cell colony-enhancing factor 1, NAmPRTase

To detect Human Visfatin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Visfatin (orb1272625) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin.

To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

This antibody stained formalin-fixed, paraffin-embedded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Quick Database Links

Gene Symbol

NAMPT

UniProt

P43490

RefSeq:P43490