Vinculin Rabbit Polyclonal Antibody

SKU: orb1294416

Description

Rabbit polyclonal antibody to Vinculin

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: Western Blot 1:2000 - 1:4000, Immunofluorescence 1:200 – 1:500, Immunohistochemistry (Paraffin) 1:200 – 1:300
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region.
• Target: Vinculin
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Disclaimer: For research use only

All lanes: Anti-Vinculin antibody - Loading Control at 1/3000 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294416 overnight at 4°C.

All lanes: Anti-Vinculin antibody - Loading Control at 1/3000 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294416 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294416 anti-Vinculin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse testis labeling Vinculin with orb1294416 at 1/300.

Quick Database Links

Gene Symbol

Vinculin