VCL Antibody

SKU: orb1240285

Description

VCL Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Vinculin antibody (orb1240285) was raised against a peptide corresponding to 23 amino acids near the amino terminus of human Vinculin.
• Target: VCL
• Molecular Weight: Predicted: 124kDObserved: 124kD
• Purification: Vinculin antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Vinculin antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CMD1W, CMH15, Metavinculin, MVCL

Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240276 (2 µg/mL), Vinculin orb1240285 (1 µg/mL), Vinculin orb1240274 (1 µg/mL), and beta-actin orb1240312 (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240285 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in PC3 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240285 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Liver Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240285 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Liver Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240285 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of Vinculin in Jurkat Cells. Immunocytochemical analysis of Jurkat cells using anti-Vinculin antibody (orb1240285) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of Vinculin in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with orb1240285 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

Quick Database Links

Gene Symbol

VCL

RefSeq (Protein):NP_003364