Vimentin (phospho Ser56) rabbit pAb

• Catalog Number: orb770382
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Vimentin (phospho-Ser56)
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser56. AA range:31-80
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 54
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-VIM antibody, anti-Vimentin antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of A549 cells using Phospho-Vimentin (S56) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Vimentin (Phospho-Ser56) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vimentin (Phospho-Ser56) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from A549 cells treated with Nocodazole 1ug/ml 16h, using Vimentin (Phospho-Ser56) Antibody. The lane on the right is blocked with the phospho peptide.