vimentin Antibody

• Catalog Number: orb1742597
• Category: Antibodies
• Description: Vimentin antibody detects vimentin, a type III intermediate filament protein with a predicted molecular weight of 54 kDa. Vimentin is highly expressed in cells of mesodermal origin as well as in some ectodermal cells, and is often used as a marker of mesenchymal stem cells. It is also utilized in combination with E-cadherin to study the morphological and phenotypic changes in cells undergoing epithelial–mesenchymal transition during development and tumor metastasis. In addition, vimentin antibody is a valuable reagent to stain neuronal precursors and developing radial glia cells.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IHC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Canine, Feline, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
• Concentration: 0.11 mg/ml (Please refer to the vial label for the specific concentration.)
• Dilution range: WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54
• Target: vimentin
• UniProt ID: P08670
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS (pH7), 1% rAlbumin, 20% Glycerol
• Alternative names: vimentin
• Note: For research use only
• Application notes: Positive control: 293T
• Expiration Date: 12 months from date of receipt.