Vimentin Rabbit Polyclonal Antibody

SKU: orb1742597

Description

Vimentin antibody detects vimentin, a type III intermediate filament protein with a predicted molecular weight of 54 kDa. Vimentin is highly expressed in cells of mesodermal origin as well as in some ectodermal cells, and is often used as a marker of mesenchymal stem cells. It is also utilized in combination with E-cadherin to study the morphological and phenotypic changes in cells undergoing epithelial–mesenchymal transition during development and tumor metastasis. In addition, vimentin antibody is a valuable reagent to stain neuronal precursors and developing radial glia cells.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, ICC, IHC, IHC-Fr, IHC-P, IP, WB
• Dilution Range: WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
• Reactivity: Canine, Feline, Human, Mouse, Rat
• Application Notes:
  Positive control: 293T

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
• Target: vimentin
• Molecular Weight: 54
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300.
• Concentration: 0.15 mg/ml (Please refer to the vial label for the specific concentration.)
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

vimentin

Quick Database Links

Gene Symbol

vimentin

UniProt

P08670