VIL1 Antibody

SKU: orb1252229

Description

VIL1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 µg/mLWestern blot: 1-2 µg/mLIHC (FFPE): 0.25-0.5 µg/mL for 30 min at RTThe stated application concentrations are suggested starting points. Titration of the Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: VIL1/1325
• Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
• Target: VIL1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VIL1, D2S1471, Villin, Villin 1, Villin-1, VIL

IHC testing of FFPE human colon with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human rectum tissue with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 1) A549, 2) HepG2 and 3) HCT-116 cell lysate with Villin antibody (clone VIL1/1325). Expected molecular weight ~93 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Villin Antibody (clone VIL1/1325). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

VIL1

UniProt

P09327