Villin Antibody

• Catalog Number: orb699523
• Category: Antibodies
• Description: Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: VIL1/4107R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 600-700 from the human protein was used as the immunogen for the recombinant Villin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Villin antibody is available for research use only.
• UniProt ID: P09327
• Storage: Store the recombinant Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Villin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon with recombinant Villin antibody (clone VIL1/4107R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon with recombinant Villin antibody (clone VIL1/4107R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.