Villin Antibody

• Catalog Number: orb386106
• Category: Antibodies
• Description: Villin (VIL1) is an epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VIL1/1325
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml for 60 min at RT,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Villin antibody is available for research use only.
• UniProt ID: P09327
• Storage: Store the Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting points. Titration of the Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human colon with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human rectum tissue with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 1) A549, 2) HepG2 and 3) HCT-116 cell lysate with Villin antibody (clone VIL1/1325). Expected molecular weight ~93 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Villin Antibody (clone VIL1/1325). Confirmation of Integrity and Purity of the Antibody.