Villin antibody

SKU: orb2276491

Description

Villin antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen. Negative Control: Colon: Villin staining should be absent in all non-epithelial cells. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-459R
• Immunogen: Recombinant fragment (around aa600-700) of human Villin protein (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VIL1; Villin-1; Villin1

Colon descendens, mucosa: Colon epithelial cells show a strong, predominantly membranous but also cytoplasmic villin immunostaining.

Kidney cortex: A strong villin positivity is seen in proximal tubuli of the kidney but not in distal tubuli or glomeruli.

Ovary Mucinous ovarian cancer showing strong villin immunostaining.

Quick Database Links

UniProt

P09327