VGLUT1/BNP1 Rabbit Polyclonal Antibody

• Catalog Number: orb1058
• Category: Antibodies
• Description: VGLUT1/BNP1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human VGLUT1/BNP1 (301-400/560aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 62 kDa
• Target: SLC17A7
• UniProt ID: Q9P2U7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: BNPI; Brain specific Na(+) dependent inorganic pho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (orb1058) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (orb1058) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (orb1058) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Human SH-SY5Y cell lysates, Lane 3: Human MCF-7 cell lysates, Primary: Anti-VGLUT1/BNP1 (orb1058) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kDa, Observed band size: 60 kDa.