VGLUT1/BNP1 Rabbit Polyclonal Antibody

SKU: orb1058

Description

VGLUT1/BNP1 Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human VGLUT1/BNP1 (301-400/560aa)
• Target: SLC17A7
• Molecular Weight: 62 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BNPI; VGLUT1; 2900052E22Rik; VGLU1_BOVIN; SLC17A7; Solute carrier family 17 member 7; VGLU1_HUMAN; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; VGLU1_MOUSE; VGLU1_RAT;

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (orb1058) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (orb1058) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with VGLUT1/BNP1 Polyclonal Antibody, Unconjugated (orb1058) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Human SH-SY5Y cell lysates, Lane 3: Human MCF-7 cell lysates, Primary: Anti-VGLUT1/BNP1 (orb1058) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kDa, Observed band size: 60 kDa.

Quick Database Links

Gene Symbol

SLC17A7

UniProt

Q9P2U7