VGLUT1 Antibody (Biotin)

SKU: orb149725

Description

Mouse monoclonal to VGlut1 (Biotin). VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate..

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N28/9 (Formerly sold as S28-9)
• Immunogen: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
• Target: VGLUT1
• Molecular Weight: 52kDa
• Purification: Protein G Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VGLUT1, Vesicular Glutamate Transporter 1, SLC17A7, BNPI, Solute Carrier Family 17 Member 7, Brain-specific Na(+)-dependent Inorganic Phosphate Cotransporter, Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 7, Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7, VGluT1, VGLUT 1

Quick Database Links

Gene Symbol

VGLUT1

UniProt

Q62634

NCBI Gene ID

116638

RefSeq (Protein):NP_446311.1