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| Catalog Number | orb1238617 |
|---|---|
| Category | Antibodies |
| Description | VGF Antibody |
| Target | VGF |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine |
| Form/Appearance | Liquid |
| Concentration | 1 mg/mL |
| Buffer/Preservatives | VGF Antibody is supplied in PBS containing 0.02% sodium azide. |
| Purification | VGF Antibody is affinity chromatography purified via peptide column. |
| Immunogen | VGF antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human VGF.The immunogen is located within the last 50 amino acids of VGF. |
| UniProt ID | O15240 |
| MW | Predicted: 68 kDa Observed: 70 kDa |
| Tested applications | ELISA, IF, IHC-P, WB |
| Application notes | VGF antibody can be used for detection of VGF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | VGF Antibody: Neurosecretory protein VGF, NERP-1 |
| Research Area | Neuroscience |
| Note | For research use only |
| NCBI | NP_003369 |

Western blot analysis of VGF expression in human brain tissue lysate with VGF antibody at (A) 1 and (B) 2 µg/ml.

Immunofluorescence of VGF in Human Brain cells with VGF antibody at 20 µg/mL.
Rat | |
46.88-3000 pg/mL | |
18.86 pg/mL |
Human | |
78.13-5000 pg/mL | |
28 pg/mL |
Mouse | |
31.25-2000 pg/mL | |
12.7 pg/mL |
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