VEGFR3 Antibody

• Catalog Number: orb1927297
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 818CT12.1.1
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2a
• Dilution range: WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 152757 Da
• Target: Purified His-tagged VEGFR3 protein was used to produced this monoclonal antibody.
• UniProt ID: P35916
• NCBI: NP_891555.2, NP_002011.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Vascular endothelial growth factor receptor 3, VEG
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

VEGFR3 Antibody western blot analysis in 293 and A549 cell line lysates (35μg/lane).This demonstrates the VEGFR3 antibody detected the VEGFR3 protein (arrow).

Immunohistochemical analysis of paraffin-embedded R. kidney section using VEGFR3. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. kidney section using VEGFR3. was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. testis section using VEGFR3. was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of HUVEC cells using VEGFR3 (green) compared to an isotype control of mouse IgG2a (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.