VEGFR3 Antibody

SKU: orb1788201

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Immunogen: Purified His-tagged VEGFR3 protein was used to produced this monoclonal antibody.
• Target: FLT4
• Molecular Weight: 152757 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FLT4;VEGFR3; Vascular endothelial growth factor receptor 3; Vascular endothelial growth factor receptor 3; Fms-like tyrosine kinase 4; Vascular endothelial growth factor receptor 3; Tyrosine-protein kinase receptor FLT4

Western blot analysis of lysates from A549, T47D cell line (from left to right), using VEGFR3 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded H. kidney section using VEGFR3. was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. testis section using VEGFR3. was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. kidney section using VEGFR3. NA was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of HUVEC cells using VEGFR3 (green) compared to an isotype control of mouse IgG2a (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

All lanes: Anti-VEGFR3 at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 153 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

FLT4

UniProt

P35916

RefSeq (Protein):NP_002011.2, NP_891555.2