VEGFR2 (FLK1/KDR) Antibody (C-term)

SKU: orb1929037

Description

VEGFR2 (FLK1/KDR) Antibody (C-term)

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research, Microbiology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IF - 1:10-50, FC - 1:10-50, IHC-P - 1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 151527 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

FLK1, VEGFR2

Western blot analysis of anti-KDR/FLK1 Pab in HeLa cell lysate. KDR (VEGFR2) (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Confocal immunofluorescent analysis of VEGFR2 (FLK1/KDR) Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

VEGFR2 (FLK1/KDR) Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

UniProt

P35968