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Description
Research Area
Images & Validation
−Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Biological activity was determined by the dose - dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0 - 8.0 ng/mL. |
| Target | VEGFA |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
Storage & Handling
−| Storage | The lyophilized VEGF165 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted VEGF165 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing VEGF165 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Biological activity was determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/mL. Blue= VEGF165 Rec Protein (Cat No: orb1230928) Pink= WHO Human VEGF Rec Protein, Green= VEGF121 Rec Protein (Animal Free) (Cat No: orb1230504)
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001028928.1 |
|---|
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Protocol Information
VEGF165 Recombinant Protein (orb1230928)
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