Human VEGF165

SKU: orb2995236

Description

Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human VEGF165(Cat#orb2995236) at 2μg/ml (100 μl/well) can bind Human VEGF-A Antibody (Bevacizumab)(Human IgG1)(Cat#orb2992936). The ED50 of Human VEGF-A Antibody (Bevacizumab)(Human IgG1)(Cat#orb2992936) is 2.20 ng/ml. (Regularly tested) Immobilized Human VEGF165 (Cat#orb2995236) at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc (Cat#orb2994454). The ED50 of Recombinant Human VEGFR1-Fc (Cat#orb2994454) is 36.4 ng/ml. (Regularly tested). BLI: Loaded Brolucizumab on AMC Biosensor, can bind Recombinant Human VEGF165 (Cat#orb2995236) with an affinity constant of 22.2 pM as determined in BLI assay. (Regularly tested) Loaded Human VEGF-A Antibody (Bevacizumab) (Cat#orb2992936) on Pro-A Biosensor, can bind Recombinant Human VEGF165 (Cat#orb2995236) with an affinity constant of 7.12 pM as determined in BLI assay. (Regularly tested) Loaded Human VEGFR1-Fc(Cat#orb2994454) on Protein A Biosensor, can bind Human VEGF165(Cat#orb2995236) with an affinity constant of 0.29 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured by its ability to induce calcineurin- NFAT signaling in 293-VEGF Res cells(Cat#Xorb3002411). The ED50 for this effect is 1.56 ng/mL.(Regularly tested)
• Tag: Tag free
• Molecular Weight: Predicted: 19.1 KDa. Observed: 18-24 KDa, reducing conditions
• Expression Region: Ala27-Arg191
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM Citrate, 8% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 4.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Vascular Endothelial Growth Factor Isoform 165; VEGF165

Quick Database Links

UniProt

P15692