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Description
Research Area
Images & Validation
−Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4 ng/mL. |
| Target | VEGFA |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses. |
| Protein Sequence | APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R |
Storage & Handling
−| Storage | The lyophilized VEGF121 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted VEGF121 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing VEGF121 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human VEGF 121 Protein [orb2994925]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 14.2 KDa. Observed: 16 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman VEGF121 Protein, hFc Tag [orb2321385]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 40.2 kDa after removal of the signal peptide. The apparent molecular mass of VEGF121-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgVEGF121 Recombinant Protein [orb1227715]
ELISA, WB
>95% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method.
15 kDa
HEK293 cells
0.05 mgVEGF 121 Recombinant Protein [orb1227837]
WB
>95% as determined by SDS-PAGE of reduced (+) and non-reduced (-) rhVEGF121.
14 kDa (monomer)
HEK293 cells
0.02 mgRecombinant human VEGF121 protein (Active, CHO) [orb3124206]
≥ 95% as determined by SDS-PAGE.
14.1 kDa
10 μg, 50 μg, 500 μg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001028928.1 |
|---|




