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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized VEGFR1/R2-Fc at 1 ug/mL can bind Human VEGF121, His Tag with a linear range of 0.2-6.2 ng/mL. |
| Target | VEGF121 |
| Tag | His Tag |
| MW | 15 kDa |
| Purity | >95% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Ala 27 - Arg 147 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human VEGF 121 Protein [orb2994925]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 14.2 KDa. Observed: 16 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman VEGF121 Protein, hFc Tag [orb2321385]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 40.2 kDa after removal of the signal peptide. The apparent molecular mass of VEGF121-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgVEGF 121 Recombinant Protein [orb1227837]
WB
>95% as determined by SDS-PAGE of reduced (+) and non-reduced (-) rhVEGF121.
14 kDa (monomer)
HEK293 cells
0.02 mgVEGF121 Recombinant Protein [orb1230504]
Greater than 98% by SDS-PAGE gel and HPLC analyses.
E. coli
0.01 mgRecombinant human VEGF121 protein (Active, CHO) [orb3124206]
≥ 95% as determined by SDS-PAGE.
14.1 kDa
10 μg, 50 μg, 500 μg

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