VEGFR2 Antibody / VEGF Receptor 2 / KDR

SKU: orb640075

Description

This MAb is specific to Mouse VEGFR2/FLK-1/CD309 and does not cross-react with FLK-2. VEGFR2 is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Endothelial cells, embryonic tissues, and megakaryocytes mainly express VEGFR2. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGFR2 include VEGF-A, VEGF-C, VEGF-D, and VEGF splice isoforms. Ligation of VEGFR2 with its ligands results in the receptor dimerization and auto-phosphorylation, stimulating endothelial cell proliferation and migration.

Research Area

Cancer, Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: Blocking, NeA, WB
• Dilution Range: Western blot (Non-reducing): 2-5ug/ml,Blocks binding of VEGF to VEGF-R2,Inhibits VEGF-induced signalingformat),Blocks tumor growth in mice
• Reactivity: Mouse
• Application Notes:
  Optimal dilution of the VEGFR2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG1, kappa
• Clone No.: DC101
• Immunogen: Recombinant full-length Mouse VEGFR2 protein was used as the immunogen for this VEGFR2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free VEGFR2 antibody (clone DC101) as confirmation of integrity and purity.

Quick Database Links

UniProt

P35918