KDR Antibody

SKU: orb1261301

Description

KDR Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human VEGF Receptor 2 (NP_002244.1).
• Target: KDR
• Molecular Weight: Observed: 240kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, FLK-1, Kinase insert domain receptor, KDR, Protein-tyrosine kinase receptor flk-1, CD309, KDR, FLK1, VEGFR2

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody (orb1261301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using VEGF Receptor 2 antibody (orb1261301) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using VEGF Receptor 2 antibody (orb1261301) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using VEGF Receptor 2 antibody (orb1261301) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

KDR

UniProt

P35968