VEGF Antibody Peroxidase Conjugated

• Catalog Number: orb345171
• Category: Antibodies
• Description: VEGF antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Conjugation: HRP
• UniProt ID: P15692
• NCBI: NP_001165097.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-VEGF Peroxidase Conjugated antibody, r
• Note: For research use only
• Application notes: This protein-A purified antibody has been tested for use in western blotting. Reactivity in other assays has not been determined. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 22 kDa in size corresponding to monomeric human VEGF-165 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western Blot showing detection of Recombinant Human VEGF-165. 50 ng of protein (Lane 1) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) 30 min at 20°C, Anti-VEGF-165 (RABBIT) Antibody Peroxidase Conjugate (p/n orb345171) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n orb348637). Arrow indicates correct 19 kDa molecular weight position expected for rH-VEGF-165.