VEGF Antibody

• Catalog Number: orb1272271
• Category: Antibodies
• Description: VEGF Antibody
• Target: Vegfa
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: Anti-mVEGF specific antibody was purified by affinity chromatography employing immobilized mVEGF matrix.
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF (murine VEGF).
• UniProt ID: Q00731
• Tested applications: ELISA, NeA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Vpf, Vegf, Vascular endothelial growth factor A, V
• Note: For research use only
• NCBI: Q00731

To detect mVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine VEGF (orb1272270) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF.

To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.