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VEGFA Antibody (Biotin)
Description
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hVEGF (human VEGF). |
| Target | VEGFA |
| Purification | Anti-hVEGF specific antibody was purified by affinity chromatography and then biotinylated. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−VEGFA Rabbit Polyclonal Antibody (Biotin) [orb452748]
IF, IHC-Fr, IHC-P, WB
Canine
Human, Rat
Rabbit
Polyclonal
Biotin
100 μlVEGFA Rabbit Polyclonal Antibody (Biotin) [orb452985]
IF, IHC-Fr, IHC-P
Canine, Equine, Human, Porcine
Rat
Rabbit
Polyclonal
Biotin
100 μl

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To detect hVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human VEGF (orb1272273) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hVEGF.

To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
VEGFA Antibody (Biotin) (orb1272272)
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