VEGFA Antibody

SKU: orb344659

Description

VEGF-A antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB: 1:333
• Reactivity: Bovine
• Application Notes:
  This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.2 kDa in size corresponding to bovine VEGF-A protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.
• Target: VEGFA
• Purity: This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine VEGF-A protein. A BLAST analysis was used to suggest cross-reactivity with VEGF-A from bovine, pig, sheep, and macaque sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against human, horse, dog, cat, or guinea pig based on 95% homology; and to rat and mouse based on 93% homology. Cross-reactivity with VEGF-A from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.51 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-VEGF-A Antibody, Vascular endothelial growth factor A, VEGF A, VEGFA, Vascular permeability factor, VPF

Western blot using Biorbyt's Protein-A Purified anti-bovine VEGF-A antibody shows detection of recombinant bovine VEGF-A at 17-19.2 kDa. Approximately 2 µg of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane. The membrane was blocked using 3% BSA diluted 1:10. The primary antibody was used at a 1:333 dilution and was incubated with the blot for 2h at room temperature. The membrane was washed and reacted with a 1:10000 dilution of IRDye™800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers. Other detection systems will yield similar results.

Quick Database Links

Gene Symbol

VEGFA

UniProt

P15691

RefSeq:27806357