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VEGF-121 Antibody
Catalog Number: orb345148
| Catalog Number | orb345148 |
|---|---|
| Category | Antibodies |
| Description | VEGF-121 antibody |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | None |
| Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human VEGFA-121 protein. A BLAST analysis was used to suggest cross-reactivity with VEGFA-121 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to VEGFA-121 from a wide variety of animals. Cross-reactivity with VEGFA-121 from other sources has not been determined. |
| Immunogen | This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human VEGFA-121 protein produced in E.coli. |
| UniProt ID | P15692 |
| Tested applications | ELISA, WB |
| Dilution range | ELISA: 1:10,000, WB: 1:1000 |
| Application notes | This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14.7 Da in size corresponding to the mature human VEGFA-121 protein by western blotting in appropriate cell lysate or extract. |
| Antibody Type | Primary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | rabbit anti-VEGF-121 antibody, rabbit anti-Vascula Read more... |
| Note | For research use only |
| NCBI | 6631029 |
Anti-human VEGF-121 by western blot shows detection of recombinant Human VEGF-121 raised in E.coli. Recombinant (0.1 µg, 14.7 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Biorbyt's Anti-Human VEGF-121. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20000 in blocking buffer (p/n orb348637) for 30 min at RT.
