VE-Cadherin, Human, mAb BV9 Antibody

SKU: orb1109032

Description

Mouse monoclonal antibody to VE-Cadherin

Images & Validation

• Tested Applications: FC, Functional Studies, IA, IF, IHC-Fr, IP, WB
• Dilution Range: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Reactivity: Human
• Application Notes:
  F: Acetone fixed sections were blocked with horse serum and incubated with antibody BV9 for 30 minutes (Ref.2). FC: Antibody BV9 stains the extracellular domain of VE-cadherin. As negative control an IgG isotype control was used (Ref.4) FS: Antibody BV9 functions as an antagonist. The antibody was functionally tested by adding 10-50µg/ml antibody BV9 to cell culture. The antibody blocks VE-cadherin causing a redistribution of VE-cadherin away from intracellular junctions(Ref.5, 6). IA: Antibody BV9 can function as coat and detector. IF: Cells on coverslips were fixed with 3% paraformaldehyde and permeabilized with 0.5% Triton X-100 before incubation with antibody BV9 (Ref.5, 8). W: A reduced sample treatment and 7.5% SDS-Page was used. The band size is 130-140kDa (Ref.3).

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.
• Buffer/Preservatives: 0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

vascular endothelial cadherin, 7B4 antigen, Cadherin-5, CD144 Gene name: CDH5