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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:2000, IF: 1:50-1:200 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-283 of human VDAC3 (NP_005653.3). |
| Molecular Weight | 31kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−VDAC3, HD-VDAC3, VDAC-3
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Immunofluorescence analysis of HeLa cells using VDAC3 Rabbit pAb (orb536944) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using VDAC3 Rabbit pAb (orb536944) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse lung using VDAC3 Rabbit pAb (orb536944) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western blot analysis of lysates from Mouse heart, using VDAC3 Rabbit pAb (orb536944) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Quick Database Links
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Filter by Applications
Filter by Species
Wei Meng 1, Linghua Li N6-methyladenosine modification of SPOP relieves ferroptosis and diabetic cardiomyopathy by enhancing ubiquitination of VDAC3 Free Radic Biol Med, (2024)
Applications
WB
Reactivity
Mouse
VDAC3 Rabbit pAb (orb536944)
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