VCP Antibody

SKU: orb1787867

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:4000, IHC-P: 1:25, FC: 1:25
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Immunogen: This VCP antibody is generated from a mouse immunized with a recombinant protein of human VCP. Antigen Region: 400-806 aa.
• Target: VCP
• Molecular Weight: 89322 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transitional endoplasmic reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, VCP, VCP

All lanes: Anti-VCP Antibody at1:4000 dilution. Lane 1: A549 whole cell lysate. Lane 2: C6 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 89 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining VCP in human breast carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).

Overlay histogram showing K562 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Quick Database Links

Gene Symbol

VCP

UniProt

P55072