VCP Antibody

SKU: orb1260450

Description

VCP Antibody

Research Area

Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 647-806 of human VCP (NP_009057.1).
• Target: VCP
• Molecular Weight: Observed: 115kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDC48, CDC48p, YDL126C, VCP, transitional endoplasmic reticulum ATPase, valosin containing protein, p97, TERA

Western blot analysis of extracts of various cell lines, using VCP antibody (orb1260450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat heart using VCP antibody (orb1260450) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using VCP antibody (orb1260450) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of C6 cells using VCP antibody (orb1260450) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using VCP antibody (orb1260450) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

VCP

UniProt

P55072