Phanta Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta Super-Fidelity DNA Polymerase. Compared with the previous generation, Phanta Max has added the unique elongation factor, specificity-enhancing factor and plateau phase anti-inhibitor factor, which greatly improves the long-fragment amplification ability, amplification specificity and amplification yield. Phanta Max can efficiently amplify up to 40 kb simple templates (e.g. λDNA, plasmids), 20 kb complex templates (e.g. genomic DNA) and 10 kb cDNA. The amplification error rate of Phanta Max is 128-fold lower than that of conventional Taq DNA Polymerase. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplification of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. This product contains Phanta Max Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. It only needs to add primers and templates to perform amplification, thereby reducing pipetting operations and improving detection throughput and reproducibility of results. The system contains protective agents that keep 2 × Phanta Max Master Mix stable in activity after repeated freezing and thawing. Amplification products are blunt-ended, which are compatible with ClonExpress kits (Vazyme #C112/C113/C116) and TOPO cloning kit (Vazyme #C603).
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