Vazyme - 2 × Phanta Max Master Mix (Dye Plus)

SKU: P525-01

Description

Phanta Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta Super-Fidelity DNA Polymerase. Compared with theprevious generation, Phanta Max has added the unique elongation factor, specificity-enhancing factor and plateau phase anti-inhibitorfactor, which greatly improves the long-fragment amplification ability, amplification specificity and amplification yield. Phanta Max canefficiently amplify up to 40 kb simple templates (e.g. λDNA, plasmids), 20 kb complex templates (e.g. genomic DNA) and 10 kb cDNA. Theamplification error rate of Phanta Max is 128-fold lower than that of conventional Taq DNA Polymerase. In addition, Phanta Max has agood resistance to PCR inhibitors and can be used for direct PCR amplification of bacteria, fungi, plant tissues, animal tissues, and evenwhole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5'→3' polymerase activity and 3'→5' exonucleaseactivity at room temperature, which enable it to perform hot start PCR with great specificity. This product contains Phanta MaxSuper-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. It only needs to add primers and templates to perform amplification, thereby reducing pipetting operations and improving detection throughput and reproducibility of results. The system contains protectiveagents that keep 2 × Phanta Max Master Mix (Dye Plus) stable in activity after repeated freezing and thawing. This product containstracking dyes, so PCR products can be directly loaded for electrophoresis after the reaction. Amplification products are blunt-ended, whichare compatible with ClonExpress kits (Vazyme #C112/C113/C116) and TOPO cloning kit (Vazyme #C603).

Images & Validation

• Application Notes:
  Ultra-high fidelity: ~128X higher than Taq DNA Polymerase Longer amplicon lengths: Up to 40 kb simple templates, 10 kb cDNA and 20 kb gDNA Robust performance: Superior specificity, sensitivity, and yields Broad Compatibility: Suitable for complex samples & GC-rich systems

Storage & Handling

• Storage: Store at -30 ~ -15°C and transport at ≤0°C.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only