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| Catalog Number | orb1272711 |
|---|---|
| Category | Antibodies |
| Description | SERPINA12 Antibody (Biotin) |
| Target | SERPINA12 |
| Clonality | Polyclonal |
| Conjugation | Biotin |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVaspin. Human Vaspin specific antibody was purified by affinity chromatography and then biotinylated. |
| UniProt ID | Q8IW75 |
| Tested applications | ELISA, WB |
| Application notes | ELISA:Sandwich:To detect hVaspin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Vaspin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin. Western Blot:To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | OL-64Serpin A12, OL-64, Vaspin |
| Research Area | Neuroscience, Signal Transduction |
| Note | For research use only |
| NCBI | Q8IW75 |

To detect hVaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Vaspin (orb1272712) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hVaspin.

To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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