VASP Antibody (C-term)

SKU: orb1936228

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:10-50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This VASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-296 amino acids from the C-terminal region of human VASP. Antigen Region: 267-296 aa.
• Target: VASP
• Molecular Weight: 39830 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Vasodilator-stimulated phosphoprotein, VASP, VASP

VASP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of VASP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-VASP Antibody (C-term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HUVEC whole cell lysate. Lane 5: MDA-MB-453 whole cell lysate. Lane 6: THP-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

VASP

UniProt

P50552

RefSeq (Protein):NP_003361.1