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VAMP2/VAMP-2 Antibody

SKU: orb1537926

Description

VAMP2/VAMP-2 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeELISA, IHC, IHC-P (10 µg/ml), WB (1:2000 - 1:3000)
ReactivityHuman
Application Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1,k
Clone No.3E5
ImmunogenRecombinant human Synaptobrevin 2 (1-89aa) purified from E. coli.
TargetVAMP2 / VAMP-2
PurificationProtein G purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS, pH 7.4, 0.1% Sodium Azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2

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VAMP2/VAMP-2 Antibody

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

VAMP2/VAMP-2 Antibody (orb1537926)

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50 μl
$ 640.00
DispatchUsually dispatched within 2-3 weeks
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