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Item 1 of 3
UVRAG Antibody: FITC
Catalog Number: orb377190
| Catalog Number | orb377190 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody against UVRAG conjugated to FITC. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ICC, IF, IHC |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide from the mid-protein of human UVRAG |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000); ICC/IF (1:100) |
| Conjugation | FITC |
| MW | 78.1kDa |
| Target | UVRAG |
| Entrez | 7405 |
| UniProt ID | Q9P2Y5 |
| NCBI | NP_003360.2 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS |
| Alternative names | DHTX antibody, p63 antibody, UV radiation resistan Read more... |
| Note | For research use only |
| Application notes | A 1:1000 dilution of SPC-606 was sufficient for detection of UVRAG on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-UVRAG Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) UVRAG Antibody (D) Composite.
Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~100kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 μg). Lane 3: Human 293T (20 μg). Load: 20 μg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~100kDa.
Western blot analysis of Rat Liver showing detection of ~100kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 μg). Load: 20 μg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~100kDa.
