UT-A1 Antibody (FITC)

SKU: orb152769

Description

Rabbit polyclonal to UT-A1 (FITC). UT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates trans-epithelial urea transport and is a target for regulation by vasopressin. Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts. (The antibody also recognizes a second protein from the UT-A gene driven by transcription from an alternative promoter and expressed in the thin descending limb of Henle, viz. UT-A2. ).

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000); IHC (1:25)
• Reactivity: Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of UT-A1 in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against a synthetic peptide mapped to the C-terminal tail (amino acids 911-929) of rat UT-A1 (antibody designation L194)
• Target: UT-A1
• Molecular Weight: 97 and 127kDa
• Purification: Peptide Affinity Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UT-A1, UT-A2, UT1, UT2, UTA, UTA1, UT2_HUMAN, UT2, UTR, SLC14A2, Slc14a2, Solute carrier family 14 member 2, Solute carrier family 14 (urea transporter) member 2, Urea transporter, Urea transporter 2, Urea transporter kidney, HUT2, hUT-A6, FLJ16167, MGC119566, MGC119567, kidney

Western blot analysis of Rat Inner medulla showing detection of UT-A1 protein using Rabbit Anti-UT-A1 Polyclonal Antibody. Primary Antibody: Rabbit Anti-UT-A1 Polyclonal Antibody at 1:1000.

Immunohistochemistry analysis using Rabbit Anti-UT-A1 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-UT-A1 Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) UT-A1 Antibody (D) Composite

Quick Database Links

Gene Symbol

UT-A1

UniProt

Q62668

NCBI Gene ID

54302

RefSeq (Protein):NP_062220