USP6 Antibody

SKU: orb1248696

Description

USP6 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in Human Testis, Prostate and Placenta lysates gave no specific signal but low background at antibody concentration up to 1µg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HHIDLDVRTTLRNH
• Target: USP6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

USP6, ubiquitin specific peptidase 6 (Tre-2 oncogene), HRP1, TRE17, TRE2, Tre-2, USP6-short, OTTHUMP00000125301, deubiquitinating enzyme 6, hyperpolymorphic gene 1, proto-oncogene TRE-2, tre-2 oncogene, ubiquitin carboxyl-terminal hydrolase 6, ubiquitin specific peptidase 6-, ubiquitin specific protease 6 (Tre-2 oncogene), ubiquitin thioesterase 6, ubiquitin thiolesterase 6, ubiquitin-specific protease USP6, ubiquitin-specific-processing protease 6

orb1248696 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic/vesicle staining.

orb1248696 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1248696 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

USP6

UniProt

P35125

RefSeq (Protein):NP_004496.2