USP25 Antibody

• Catalog Number: orb1238623
• Category: Antibodies
• Description: USP25 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25.The immunogen is located within amino acids 470 - 520 of USP25.
• Concentration: 1 mg/mL
• Dilution range: USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 116 kDa Observed: 105 kDa
• Target: USP25
• UniProt ID: Q9UHP3
• NCBI: NP_037528
• Storage: USP25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: USP25 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: USP25 Antibody: USP21, USP21, Ubiquitin carboxyl-t
• Note: For research use only
• Application notes: USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 µg/mL.

Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 µg/mL.