USP2 Antibody (C-term L523)

SKU: orb33724

Description

Purified rabbit polyclonal antibody.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: IHC-P: 1:50-100, WB: 1:2000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD6639
• Immunogen: Synthetic Peptide
• Target: USP2
• Molecular Weight: 68072
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Ubiquitin carboxyl-terminal hydrolase 2 antibody, anti-Ubiquitin thiolesterase 2 antibody, anti-Ubiquitin-specific processing protease 2 antibody, anti-Deubiquitinating enzyme 2 antibody, anti-41 kDa ubiquitin-specific protease antibody, anti-UBP41 antibody

Anti-USP2 Antibody (L314) at 1:2000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human testis tissue reacted with USP2 antibody (C-term L523), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Staining USP2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/100) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

USP2

UniProt

O75604

RefSeq (Protein):NP_741994.1, NP_004196.4, NP_001230688.1

Davis, Mindy I. et al. Small Molecule Inhibition of the Ubiquitin-specific Protease USP2 Accelerates cyclin D1 Degradation and Leads to Cell Cycle Arrest in Colorectal Cancer and Mantle Cell Lymphoma Models J Biol Chem, 291, 24628-24640 (2016)

PubMed: 27681596