USP17L24 Antibody (C-term)

• Catalog Number: orb1930477
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB26955
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:500, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 59711 Da
• Target: This USP17L24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-524 amino acids from the C-terminal region of human USP17L24.
• UniProt ID: Q0WX57
• NCBI: NP_001229259.1, NP_001229255.1, NP_001229261.1, NP_001243796.1, NP_001229260.1, NP_001229257.1, NP_001229256.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ubiquitin carboxyl-terminal hydrolase 17-like prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

USP17L24 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded Human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-251 MG cells labeling USP17L24 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus staining on U-251 MG cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (red). The nuclear counter stain is DAPI (blue).

USP17L24 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the USP17L24 antibody detected the USP17L24 protein (arrow).

Western blot analysis of lysate from MAD-MB-231 cell line, using USP17L24 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

All lanes: Anti-USP17L24 Antibody (C-term) at 1:1000 dilution. Lane 1: U-87 MG whole cell lysate. Lane 2: MDA-MB-231 whole cell lysate. Lane 3: U-251 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.