USP15 Antibody (N-term)

SKU: orb1931688

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:25, WB - 1:1000, IHC-P-Leica - 1:2500
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This USP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the N-terminal region of human USP15. Antigen Region: 151-180 aa.
• Target: USP15 {ECO:0000303|PubMed:10444327, ECO:0000312|HGNC:HGNC:12613}
• Molecular Weight: 112419 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4, USP15, KIAA0529

Immunohistochemical analysis of paraffin-embedded human liver tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human epityphlon tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/2500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela cells labeling USP15 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm and Weak Nucleus staining on Hela cell line. The nuclear counter stain is DAPI (blue).

The anti-USP15 Pab is used in Western blot to detect USP15 in mouse brain tissue lysate.

Anti-USP15 Antibody (N-term) at 1:1000 dilution + MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 112 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

USP15 {ECO:0000303|PubMed:10444327, ECO:0000312|HGNC:HGNC:12613}

UniProt

Q9Y4E8

RefSeq (Protein):NP_001239008.1, NP_001239007.1, NP_006304.1