USP14 Antibody (N-term)

• Catalog Number: orb1788306
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: 1-474 aa
• Dilution range: WB: 1:2000, WB: 1:1000, FC: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 56069 Da
• Target: Purified His-tagged USP14 protein was used to produced this monoclonal antibody.
• UniProt ID: P54578
• NCBI: NP_005142.1, NP_001032411.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: USP14;TGT; Ubiquitin carboxyl-terminal hydrolase 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from K562 cell line, mouse brain, rat brain tissue, Jurkat cell line (from left to right), using USP14 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

All lanes: Anti-USP14 Antibody (N-term) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: mouse brain whole cell lysate. Lane 3: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Jurkat cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.