Urokinase (PLAU) Antibody (C-term)

• Catalog Number: orb1928539
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Urokinase (PLAU) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-426 amino acids from the C-terminal region of human Urokinase (PLAU).
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P00749
• MW: 48523 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB04089-04090
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Urokinase-type plasminogen activator, U-plasminoge
• Note: For research use only
• NCBI: NP_001138503.1, NP_002649.1

Western blot analysis of anti-PLAU Pab in CEM cell line lysate (35 ug/lane). PLAU (arrow) was detected using the purified Pab

Confocal immunofluorescent analysis of Urokinase (PLAU) Antibody (C-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Urokinase (PLAU) Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PLAU antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.