UREA_CANEN Antibody (HRP)

SKU: orb344911

Description

Urease antibody (Peroxidase)

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution Range: ELISA: 1:500 - 1:2,000, WB: 1:500
• Reactivity: Plant
• Application Notes:
  Anti-Urease peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Urease [Jack Bean] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Urease [Jack Bean]
• Target: UREA_CANEN
• Purity: Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Urease Antibody HRP Conjugation, Peroxidase Conjugated rabbit anti-Urease Antibody, Urea Amidohydrolase antibody

Dot Blot Results of Rabbit Anti-Urease Antibody Peroxidase Conjugated. Urease (1) 33.33 ng, (2) 11.11 ng, (3) 3.70 ng, (4) 1.23 ng. Primary Antibody: Anti-Urease HRP at 1.0 µg/ml for 1hr at RT. Secondary Antibody: none. Blocking: BlockOut Buffer (orb348644) for 30 mins RT. Exposure: 5 secs.

Quick Database Links

Gene Symbol

UREA_CANEN

UniProt

P07374

RefSeq:AAA83831.1