UQCRFS1 Antibody (C-term)

SKU: orb1938042

Description

UQCRFS1 Antibody (C-term)

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Microbiology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Gallus, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 29668 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Confocal immunofluorescent analysis of UQCRFS1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

UQCRFS1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-UQCRFS1 Antibody (C-term) at 1:1000 dilution + Raji whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

UQCRFS1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UQCRFS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

UQCRFS1 Antibody (C-term) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the UQCRFS1 antibody detected the UQCRFS1 protein (arrow).

Quick Database Links

UniProt

P47985